Iseli et al. disclosed the presence of bufogenins in Bufo asper Gravenhorst by paper chromatography (l), but isolation of these bufogenins and investigation on bufotoxins (2) from this toad venom have not been done. Here we report the isolation of five bufogenins (resibufogenin, cinobufagin, bufalin, bufotalin, marinobufagin) and three bufotoxins (resibufotoxin, bufalitoxin, vulgarobufotoxin) from the skin of this toad.