Three new monotetrahydrofuran annonaceous acetogenins, Annosquamins A–C (1–3) were isolated from the 95% EtOH extract of Annona squamosa seeds, in addition to the known compounds, solamin, annotemoyin-1, uvariamicin-II, uvariamicin-III. Their structures were elucidated by spectroscopic analysis and the relative configurations of bearing tetrahydrofuran rings were established by comparing NMR data with model compounds.