Actinomycetes have evolved in the process of ecological interactions between animals, plants and microorganisms in the environment to produce new classes of secondary metabolites with novel structures,1–3 of which polyene macrolides are very interesting bioactive compounds with a wide range of biological activities and subject to a relatively low incidence of resistance.4,5 Many of these molecules have been successfully isolated and turned into useful drugs and other organic chemicals. In our study aimed at the discovering of novel antifungals, the soil actinomycete, Streptomyces lavenduligriseus, was found to produce strong antifungal components. Bioactivity-guided isolation and purification yielded filipin III 1 and three novel polyene macrolides, compound 2, 3 and 4 (Figure 1). Details of the isolation, structure elucidation and the antifungal activities of these compounds are presented below.