Metabolites from Phoma sp., a phytopathogenic fungus, were exploited for their herbicidal potential. As part of our search for potential herbicidal agents, presently we describe the isolation, purification, and characterization of a pigment exhibiting herbicidal potential against the prominent weeds of Central India. The phytotoxic compound isolated was identified as anhydropseudophlegmacin-9,10-quinone-3-amino-8-Omethyl ether on the basis of spectral data. The isolated pigment was tested for its herbicidal potential against the prominent weeds of Central India, where a positive result was obtained. This is the first report where a pigment, i.e., anhydropseudophlegmacin-quinone, exhibits herbicidal potential against the target weeds.