<jats:title>Abstract</jats:title><jats:p>From the roots of <jats:italic>Pseudostellaria heterophylla</jats:italic>, three cyclopeptides and three amides were isolated, besides heterophyllin A and B. Their structures were determined as cyclo (Ala‐Gly‐Pro‐Val‐Tyr‐) (heterophyllin J; <jats:bold>1</jats:bold>), cyclo (Ala‐Gly‐Pro‐Tyr‐Leu‐) (pseudostellarin A; <jats:bold>2</jats:bold>), cyclo (Gly‐Gly‐Gly‐Pro‐Pro‐Phe‐Gly‐Ile‐) (pseudostellarin B; <jats:bold>3</jats:bold>), methyl <jats:italic>γ</jats:italic>‐hydroxypyroglutamate (<jats:bold>4</jats:bold>), methyl pyroglutamate (<jats:bold>5</jats:bold>), and pyroglutamic acid (<jats:bold>6</jats:bold>) on the basis of spectral data, especially 2D‐NMR data. Among them, compounds <jats:bold>1</jats:bold> and <jats:bold>4</jats:bold> are new compounds.