The sole glucosinolate in the seed-bearing portion of Lepidium bonariense L., collected in Queensland, is shown to be p-methoxybenzylglucosinolate (IV). Seed material of L. hyssopifolium Desv., collected in Queensland, contains solely 3,4,5-trimethoxybenzylglucosinolate (V). A previous report on the presence of benzylglucosinolate in L. hyssopifolium is discussed.