During the course of our screening for novel and potent antifungal agents active against phytopathogenic fungi, we isolated two new members of azalomycin class, 2-demethylazalomycin F4a (1) and 2-demethylazalomycin F5a (2) from the fermented broth of an Actinomycete sp. HIL Y-9120362. Herein, we report the production, isolation, structure elucidation and biological properties of 1 and 2.