Two new sesquiterpene lactone glycosides and three new kaurene glycosides closely related to carboxyatractyloside and atractyloside, together with the 3', 4'-didesulphated-carboxyatractyloside and 3', 4'-didesulphatedatractyloside, were isolated from the aerial parts of Xanthium spinosum. Their structures were established by spectroscopic methods (¹H, ¹³C, DEPT ¹³C NMR and FAB mass spectr).