Bioassay-guided fractionation of a methanol extract of the fruits of Eucalyptus camaldulensis Dehnh. with moderate antiproliferative activity afforded the new triterpene, 3b-acetoxy-urs-11,13(18)-dien-28-oic acid (1) along with the known triterpenoids 3b-hydroxy-urs-11-en-28,13b-olide (2), 3b-acetoxy-urs-11-en-28,13b-olide (3), 3-acetylbetulinic acid (4), oleanolic acid (5), ursolic acid (6), b-amyrin acetate (7), b-sitosterol (8) and sitosterol 3-O-b-D-glucopyranoside (9). Their structures were established on the basis of analysis of their 1 H and 13C NMR, APT, gHSQC, gHMBC, UV, IR and mass spectral data. The tested triterpenoids (1–7) exhibited weak-moderate antiproliferative activity against the A2780 human ovarian cancer cell line.