<jats:sec><jats:title>Abstract</jats:title><jats:p>Chemical examination of the ethyl acetate extract from the fermentation broth of the marine‐derived bacterium <jats:italic>Bacillus licheniformis</jats:italic> resulted in the isolation of two new glycolipids, ieodoglucomide C (<jats:bold>1</jats:bold>) and ieodoglycolipid (<jats:bold>2</jats:bold>). The structural characterization of <jats:bold>1</jats:bold> and <jats:bold>2</jats:bold> was achieved by extensive spectroscopic evidence, including 2D NMR experiments. A combination of chemical derivatization techniques followed by NMR studies, LC‐MS data analysis and a literature review was deployed for the establishment of the stereo‐configurations of <jats:bold>1</jats:bold> and <jats:bold>2</jats:bold>. Compounds <jats:bold>1</jats:bold> and <jats:bold>2</jats:bold> exhibited good antibiotic properties against <jats:italic>Staphylococcus aureus</jats:italic>, <jats:italic>Bacillus subtilis</jats:italic>, <jats:italic>Bacillus cereus</jats:italic>, <jats:italic>Salmonella typhi</jats:italic>, <jats:italic>Escherichia coli</jats:italic> and <jats:italic>Pseudomonas aeruginosa</jats:italic> with MICs ranging from 0.01 to 0.05 μM. Furthermore, the antifungal activity of <jats:bold>1</jats:bold> and <jats:bold>2</jats:bold> was evaluated against plant pathogenic fungi <jats:italic>Aspergillus niger</jats:italic>, <jats:italic>Rhizoctonia solani</jats:italic>, <jats:italic>Botrytis cinerea</jats:italic> and <jats:italic>Colletotrichum acutatum</jats:italic> as well as the human pathogen <jats:italic>Candida albicans</jats:italic>. Compounds <jats:bold>1</jats:bold> and <jats:bold>2</jats:bold> inhibited the mycelial growth of these pathogens with MIC values of 0.03–0.05 μM, revealing that these compounds are good candidates for the development of new fungicides.</jats:sec>