Three new 3-prenylindoles named paniculidines A, B and C were isolated from the root bark of Murraya paniculata together with the known coumarins murralongin and osthol. Their structures were elucidated as methyl 2- (R)-methyl-4-(3-indolyl)-butyrate, 2-(R)-methyl-4-(1-methoxy-3-indolyl)-1-butanol and 2-(R)-methyl-4-(3-indolyl)-lbutanol, respectively, based on the spectroscopic and chemical evidence. The chemotaxonomic and biogenetic aspects of 3-prenylindole in Murraya has been discussed in conjunction with carbazole biogenesis.