Three new kendomycin analogues, kendomycins B-D (<b>1</b>-<b>3</b>), were discovered from the marine-derived actinomycete <i>Verrucosispora</i> sp. SCSIO 07399. The structures of <b>1</b>-<b>3</b> were elucidated using diverse spectroscopic data analyses, X-ray crystallography, and semisynthetic derivatization. <i>In vitro</i> antimicrobial assays revealed that <b>1</b>-<b>3</b> all display good antibacterial activities against six Gram-positive bacteria with MIC values ranging from 0.5 to 8.0 μg/mL. Additionally, <b>1</b>-<b>3</b> were found to be moderately cytotoxic against MGC803, A549, HeLa, HepG2, MCF-7, and RKO human tumor cell lines; IC<sub>50</sub> values ranged from 2.2 to 44 μM.