Steam distillation, solvent extraction, supercritical fluid extraction (SCCO2) and liquid CO2 extraction were used to obtain the volatile oil from Western Australian Sandalwood (Santalum spicatum (R. Br.) A. DC.). Supercritical fluid extraction afforded the highest yields of extractable material and total volatiles. The percentages of five sesquiterpene alcohols, epi-α-bisabolol (1), (Z)-α-santalol (2), 2(E), 6(E)-farnesol (3), (Z)-β-santalol (4) and (Z)nuciferol (5), were highest in the steam distillate. The variations in the relative amounts of these sesquiterpenes in the essential oil recovered by SCCO2 extraction of different sections of a single tree have been investigated.