The new antibiotic, moenomycin, isolated from Streptomyces bambergiensis is a mixture of several, chemically very similar components, which are particularly characterized by their phosphorus content. The moenomycin complex can be separated into components A, B₁, B₂ and C by means of column chromatography on silica gel with n-propanol - 2n ammonia as eluant. The use of other chromatographic procedures, e. g. chromatography on anion exchange resins, results in a further separation of fraction B₁ into components D, E and F; similarly fraction B₂ can be separated into components G and H. Isolation and properties of moenomycins D, E, F, G and H are described.