Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli

Analytical Biochemistry
2005.0

Abstract

Undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc (lipid II) is extracted from Escherichia coli cells by utilizing its unusual pH-dependent solubility property in a Bligh-Dyer system, and identified by electrospray ionization mass spectrometry in conjunction with a novel 15N mass shift analysis. The described approach will facilitate the structural characterization of lipid II variants from diverse bacteria, including antibiotic-resistant mutants, as well as the numerous minor uncharacterized lipids present in all biological systems.

Knowledge Graph

Similar Paper

Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli
Analytical Biochemistry 2005.0
Isolation and characterisation of 3-deoxy-d-manno-2-octulopyranosonate 7-(2-aminoethyl phosphate) from the inner core region of Escherichia coli K-12 and Salmonella minnesota lipopolysaccharides
Carbohydrate Research 1990.0
Structure elucidation of liposidomycins, a class of complex lipid nucleoside antibiotics
The Journal of Organic Chemistry 1992.0
Identification and structural elucidation of new caprazamycins from <i>Streptomyces</i> sp. MK730–62F2 by liquid chromatography/electrospray ionization tandem mass spectrometry
Rapid Communications in Mass Spectrometry 2011.0
Synthesis and biological evaluation of analogues of bacterial lipid I
Bioorganic &amp; Medicinal Chemistry Letters 2000.0
Total Synthesis of Laspartomycin C and Characterization of Its Antibacterial Mechanism of Action
Journal of Medicinal Chemistry 2016.0
Identification of an ornithine-containing lipid from Cytophaga johnsonae Stanier strain C21 by 1H-NMR
Chemistry and Physics of Lipids 1996.0
Tandem mass spectrometry methods to accelerate the identification of phytotoxic metabolites produced by<i>Streptomyces</i>sp. 39 PL
Natural Product Research 2020.0
Pholipeptin, a Novel Cyclic Lipoundecapeptide from<i> Pseudomonas fluorescens</i>
The Journal of Organic Chemistry 1997.0
Isolation and structural analysis of bamylocin A, novel lipopeptide from Bacillus amyloliquefaciens LP03 having antagonistic and crude oil-emulsifying activity
Archives of Microbiology 2007.0