<jats:p>Together with bacteriohopanetetrol, which is identical to bacteriohopanetetrol isolated from <jats:italic>Bacillus acidocaldarius</jats:italic>, three other bacteriohopane derivatives were isolated from the methylotrophic bacterium <jats:italic>Methylobacterium organophilum</jats:italic>. Three different kinds of polar moieties were found linked to the C‐35 hydroxyl group of bacteriohopanetetrol: glucosamine linked through a glycosidic bond and two new polyhydroxylated methylcyclopentanoids differing one from each other by the presence of an amino or a guanidino group and linked through an ether bond. The significance of the presence of these compounds in terms of membrane reinforcement and DNA/triterpenoid interactions is discussed.