Refined separation procedures have facilitated not only the purification of the known eudistomins G, H, I and P, but also the isolation and identification of three new B-carbolines, eudistomins R, S, and T. Excellent resolution of the closely related eudistomins was achieved by use of an amino bonded phase HPLC column: structure elucidation was accomplished by spactroanalytical methods.