Isatis tinctoria L. (Brassicaceae) was used for centuries to produce the blue dye indigo due to its indigo precursors isatan B and indican. Recently, we reported on fungicidal and insecticidal compounds (1), which explain the wood preservative properties (2) of the sap of I. tinctoria. Ficht et al. (3) described the successful use of its leaves as a wound and allergic prophylaxis in the form of tea preparations. Only a few chemical investigations have been made on the chemical composition of I. tinctoria (see, e.g., 4—8). Our study on the organic phase of its leaves resulted in the isolation of the fungicidal and insecticidal compounds tryptanthrin, indole-3-acetonitrile, and p- coumaric acid methyl ester (9). The present research on the leaves was carried out to identify further compounds, especially glycosides, contributing to the plant's pharmacological effects. We isolated one novel anthranilic acid derivative N-carbamoylanthranilic acid methyl ester (1) and twelve known compounds [two β-phenylethyl glycosides: martynoside (2) (10), β-phenylethyl-β-D-glucopyranoside (3) (11); four ionone derivatives: roseoside (4) (12), 3-oxo-α-ionyl-β-D-glucopyranoside (5) (13), icariside B2 (6) (14), blumenol C-β-D-glucopyranoside (7) (11); one lignan compound: icariside F4 (8) (15); one phenol glycoside: 4-hydroxy-2-methoxy-phenyl-β-D-glucopyranoside (9) (16); two nucleosides: adenosine (10), uridine (11) (4); anthranilic acid (12) and its derivative: N-formylanthranilic acid methyl ester (13) (17).