A new antibiotic fumaramidmycin produced by Streptomyces kurssanovii NR-7GG1 was isolated as colorless crystals. It is a new metabolite produced by agar cultures of the strain, possessing an antimicrobial activity against Gram-positive and Gram-negative bacteria. The structure was shown to be N-(phenylacetyl) fumaramide. Starting from fumaramic acid, fumaramidmycin has been synthesized in good yield, in which the key stage involves N-acylated imino ether formation followed by mild acid hydrolysis. Five analogues of fumaramidmycin have also been prepared. This paper deals with the isolation, characterization, structure determination and synthesis of fumaramidmycin as well as the preparation of its analogues.