From the rhizome of Iris germanica one new hexaoxygenated isoflavone, two new polyoxygenated isoflavone glucosides, the known isoflavonoids irisolidone, irigenin and iridin and acetovanillone, sitosterol, α-amyrin and β-amyrin were isolated. The structures of the new compounds were established by chemical and spectroscopic means and by correlation with known constitutents.