Global pollution from excessive pesticide use has become a serious environmental and public health problem. The aim of the study was to optimize the fungal mediated simultaneous removal of carbofuran and carbaryl from soil. Carb-PV5 strain was isolated from contaminated soil following enrichment culture technique; based on 18S rRNA sequencing, strain was identified as Acremonium sp. (MK514615); Field Emission Scanning Electron Microscopic analysis reflected its morphology. Towards the development of bioaugmentation strategy for the bioremediation of carbamate-contaminated soil, the process parameters were optimized employing Central Composite Rotatable Method. The experimental studies were performed in the range of biomass (0.2-0.6 g kg