Six new compounds, penicitrinols L–O (1–4), penicillenols D1 (9) and D2 (10), together with six known compounds were isolated from the marine-derived fungus Penicillium citrinum. Their structures were elucidated on the basis of comprehensive spectral analysis and chemical methods. All the new compounds were evaluated for their cytotoxic effects on the A-549, HL-60 and SW-620 cell lines by the MTT method. Penicitrinols L (1) and M (2) showed weak cytotoxicities against SW-620 cell line, while penicillenols D1 (9) and D2 (10) showed weak cytotoxicities against A-549 and HL-60 cell lines, respectively.