The result of the isolation and identification of 12 ergoline alkaloids from seeds of Stictocardia campanulata (Convolvolaceae) ; viz. : lysergol, elymoclavine, ergometrine, penniclavine, isopenniclavine, ergometrinine, isolysergol, chanoclavine-I, ergine, chanoclavine-11, a-dihydrolysergol and isoergine by using column chromatography combined with two dimensional thin layer chromatography procedures are reported.The spectrophotometric and spectrodensitometric assay of total and individual ergoline alkaloids are given.