In continuation of our work (1,2) on the isolation of diosgenin, 5α-stigmast-9(11)-en-3β-ol, saponins, and aliphatic constituents from the roots of Costus speciosus Sm. (Costaceae), we now report the isolation of four cycloartane derivatives. One of the compounds 31-norcycloartanone is a new natural product although it has been prepared synthetically (3—5) while the isolation of other three compounds constitutes the first report from this plant. The plant material was obtained from our Experimental Farm, Kukrail and a voucher specimen has been deposited in the Botany Department. The n-hexane extract (12.8 g) from air-dried, powdered roots (2 kg) was subjected to column chromatography on silica gel. Fractions of hexane-benzene (4: 1) furnished a solid which was crystallized from acetone to give compound 1. A mixture of compounds 2, 3, and 4 was obtained from the hexane-benzene (7:3) fractions. Compound 1 was identified as 31-norcycloartanone by spectral data. Its identity was further confirmed by comparison of the data of its sodium borohydride reduction product to that of 31-norcycloartanol (6, 7). The mixture of compounds 2, 3, and 4 was acetylated with Ac₂O/pyridine and resolved by GC-MS analysis.