Carcinogen in rock fern (Cheilanthes sieberi) from New Zealand and Australia

Australian Veterinary Journal
1989.0

Abstract

Bracken fern (Pteridium spp) is known to cause several ruminant diseases including neoplasia. In Australia, the distribution of bovine enzootic haematuria exceeds that of Pteridium esculentum and coincides with rock fern (Cheilanthes sieberi), suggesting C. sieberi as a potential cause. While C. sieberi feeding induced acute bracken-like poisoning in cattle, its role in enzootic haematuria was unconfirmed. This study investigated ptaquiloside (a major bracken carcinogen) in C. sieberi from New Zealand and Australia. Samples were sieved, water-extracted, cleaned, and analyzed for ptaquiloside via HPLC (with confirmatory conversion to pterosin-B). New Zealand C. sieberi had high ptaquiloside in croziers, leaves, and stems (not spores). Northern Australian samples showed high levels, but a 5-year-old composite sample had none (likely due to age/storage). Results support C. sieberi as a cause of bovine enzootic haematuria in some Australian areas and ptaquiloside as its toxic/carcinogenic agent; C. sieberi is sparse in New Zealand and non-toxic to livestock there. Additionally, Barr et al. (1986) reported an H7N7 avian influenza outbreak in Victoria, Australia, with the virus highly pathogenic to chickens/turkeys. This study describes lesions in intranasally infected chickens: 6- and 12-week-old birds inoculated with 1:10, 1:1000, or 1:100000 diluted allantoic fluid showed 22/24 infection (8 deaths, 14 euthanizations). Clinical signs included depression, dullness, and swollen heads; gross lesions were head oedema (combs/wattles) and pancreatic mottling; histopathology revealed necrosis in the bursa of Fabricius, pancreas, and brain, plus brain perivascular cell proliferation.

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