Chelidonium majus L. (Papaveraceae) is a well-known and widely investigated medicinal plant, and its alkaloids are mainly responsible for pharmacological activities. In this work, we report the separation and identification of two other aporphine alkaloids, corydine and norcorydine, from the roots of C. majus. The plant material was collected on 5th June 1996 from Golestan national park, north east of Iran, identified by the Department of Botany of the Medical Sciences University of Tehran, and a specimen (No. 6506) was deposited in the herbarium of the Faculty of Pharmacy. The methanolic perculated extract of the roots (300 g) was evaporated under reduced pressure, the residue was treated with acetic acid solution (50% W/V in water) to adjust pH to 2—3, extracted with petroleum ether, the aqueous layer was made alkaline with ammonia (25% W/V in water) and extracted with chloroform to give 4.26 g of crude mixture of alkaloids. This material was fractionated on a silica gel column (4.5 x 80cm) eluted with consecutive solvent systems including petroleum ether, chloroform-petroleum ether, chloroform, methanol-chloroform, and methanol, yielding alkaloids chelidonine (yield 0.73%), stylopine (yield 0.28%), protopine (yield 0.89%), 8-acetonyldihydrosanguinarine (yield 0.03%), corydine (eluted with MeOH-CHCI3 4:6, yield 0.02%), and norcorydine (eluted with MeOH-CHCI3 5:5, yield 0.03%). Corydine and norcorydine are reported for the first time for C. majus. Identification of the alkaloids was carried out by IR, UV, 1H-NMR and mass spectral comparison.