Three alkaloids, namely cocsulin, cocsolin and pendulin, have been isolated from the roots of Cocculus leaebe (C. pendulus). Anticancer activity has been observed in the leaves and stems of C. leaebe [1] (Menispermaceae). Eleven Alkaloids, namely columbine, palmatine, coclaurine, menisarine, sinactine, pendulin, cocsolin, cocsulin, pendulinin, pendin and cocsulinin, have been isolated from the leaves and stems of C. leaebe [2, 3,4,5]. Of these only cocsulinin was found to be active against cells derived from human epidermoid carcinoma of the nasopharynx in vitro [5]. The present programme of screening of Pakistani plants for anticancer activity led to the observation of anticancer activity in the roots of C. leaebe (EDS() = 30 Ag/mI). Search of the literature revealed that no chemical work has been done on the roots of the plant. During the present work only three alkaloids, namely cocsulin, cocsolin and pendulin, could be isolated from the active fraction of the roots. TLC indicated the presence of other minor alkaloids which could not be isolated. The identity of the compounds was established by comparison of the isolated compounds with authentic samples. The yield of these alkaloids from the roots is more than that reported from the stems and leaves [4].