Erythromycin A N-oxide and pseudoerythromycin A hemiketal, accompanying erythromycin A and erythronolide B, were isolated from the fermentation broth of Saccharopolyspora erythraea by combination of thin-layer chromatography on silica gel and reversed-phase high-performance liquid chromatography. Their identification is based on the mass and ¹H and ¹³C NMR spectrometric results.