Two new alkaloids of conanine series, holamide (N-(N'-methylacetamido)-3-0x0-1,4-conadiene) (I), pubescinine (11~-acetoxy-N-demethyl-3-oxo-1,4,17(20)-conatriene) (2) and a known alkaloid norconessine (3-N-methylamino-5-conanene) (3), have been isolated from the bark of Holarrhena pubescens Buch. Ham (Holarrhena antidysenterica Linn.) (Apocynacea). Their structures have been established through spectroscopic studies. Both 1 and 2 showed hypotensive activity in rats at a dose of 3 mglkg.