Four new b-carbolines 1–4 and two new indoles 5–6 were isolated together with thirteen known compounds from actinomycete, Actinomadura BCC 24717. Structures of the new compounds, 1–6, were determined by NMR spectroscopic and MS spectrometric analyses. Compound 4 exhibited cytotoxicity to Vero cells and compound 6 showed antifungal activity with IC50 35.91 mg/mL and 41.97 mg/mL, respectively.