Isolation method for the purpose of obtaining kinds of actinomycetes in plant leaves has been devised. All isolates were classified into genus based on their taxonomical characteristics. They included not only the genus Streptomyces but also the rare genera. In particular, strains belonging to the genus Microbispora represented more than 50% of the total share belonging to the rare isolates. Two strains of isolates produced new herbicidal antibiotics, herbicidin H produced by Streptomyces sp. SANK 63997 and γ-glutamylmethionine sulfoximine by Microbispora sp. SANK 62597.