Literature

Abstract

Heterologous expression of large fragments of microbial DNA extracted directly from environmental samples (environmental DNA, or eDNA) in easily cultured hosts should provide access to some of the natural products produced by previously uncultured bacteria. The natural product antibiotic palmitoylputrescine (1) was isolated from Escherichia coli transformed with a cosmid (pCSLF16) containing DNA extracted directly from Costa Rican bromeliad tank water. In this report we describe the characterization of this antibiotic and its biosynthetic gene.

DOI： 10.1021/np0499766

Palmitoylputrescine, an Antibiotic Isolated from the Heterologous Expression of DNA Extracted from Bromeliad Tank Water

Journal of Natural Products 2004.0

N-Acyl Derivatives of Arginine and Tryptophan Isolated from Environmental DNA Expressed in Escherichia coli

Organic Letters 2005.0

Pantocin B, an Antibiotic from Erwinia herbicola Discovered by Heterologous Expression of Cloned Genes

Journal of the American Chemical Society 1999.0

Structural and Functional Analysis of Pantocin A: An Antibiotic from Pantoea agglomerans Discovered by Heterologous Expression of Cloned Genes

Angewandte Chemie International Edition 2003.0

Fluostatins Produced by the Heterologous Expression of a TAR Reassembled Environmental DNA Derived Type II PKS Gene Cluster

Journal of the American Chemical Society 2010.0

In Situ Activation and Heterologous Production of a Cryptic Lantibiotic from an African Plant Ant-Derived Saccharopolyspora Species