Three new steroidal alkaloids, regholarrhenine D, E and F, along with the known alkaloid kurchessine, were isolated from the stem bark of Holarrhena antidysenterica. The structures were elucidated on the basis of spectral and chemical evidence. It was shown that the first alkaloid possesses the endo N-OH function in lieu of the endo N-Me function in conessine. The second alkaloid is the C-7 stereoisomer of the previously isolated alkaloid kurcholessine. The third alkaloid was established as the 18-hydroxynlethyl analogue of kurchessine. © 1990.