Two novel cyclic hexapeptides, comoramides A and B and two new cyclic heptapeptides, mayotamides A and B were isolated, separately, from two collections of the compound ascidian Didemnum raolle collected at two spots of the lagoon of Mayotte. The structure of all four peptides, including the absolute configuration of all amino adds, was elucidated by spectroscopic analysis, mainly NMR data, and degradation experiments. All four compounds exhibit mild eytotoxicity against several tumor cells. Obtaining four new cyclic peptides from two D. molle collections, which add to the two earlier reported cyclic peptides from an Australian and Philippine D. molle, supports the notion that these compounds originate from prokaryotic algal symbionts.