Three new hopane saponins, lotoidosides A - C(1-3), and four new oleanane saponins, lotoidosides D - G(4-7), were isolated from the roots of Glinus lotoides L. (Molluginaceae). Their structures were determined by extensive 1D- and 2D-NMR spectroscopy and ESI-MS analysis. The antiproliferative activity of compounds 1-7 was evaluated using three continuous murine and human culture cell lines J774.A1, HEK-293, WEHI-164. Compounds 1 and 3-7 showed significant cytotoxicity against the three cancer cell lines with IC₅₀ values ranging from 0.018 to 0.62 μM, while compound 2 exerted a weak activity only against the cell line HEK-293.