The cyclic peptide alkaloid, chamaedrine, was isolated from the roots of Melochia chamaedris (Sterculiaceae), along with four known cyclic peptide alkaloids (adouetine X, frangulaline, scutianine B and scutianine C), and waltherione A, parasorbic acid, propacine, and (-)-epicatequine. Their structures were elucidated on the basis of spectroscopic analysis, especially by 2D NMR ((1)H-(1)H-COSY, NOESY, HMQC, HMBC).