<jats:title>ABSTRACT</jats:title> <jats:p> Two <jats:italic>Bacillus subtilis</jats:italic> genes encoding two proteins (currently annotated ThiD and YjbV) were overexpressed and characterized. YjbV has 4-amino-5-hydroxymethyl-2-methylpyrimidine and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase activity and should be reannotated ThiD, and <jats:italic>B. subtilis</jats:italic> ThiD has pyridoxine, pyridoxal, and pyridoxamine kinase activity and should be reannotated PdxK.