A new lupane-type nortriterpene and 13 known compounds were isolated from the leaves of Melaleuca leucadendron L. Based on chemical and spectral methods, the structure of the new compound was elucidated as 28-norlup-20(29)-ene-3beta,17beta-diol, while the known compounds were identified as (2E,6E)-farnesol, phytol, squalene, alloaromadendrene, ledene, palustrol, viridiflorol, ledol, betulinaldehyde, betulinic acid, 3beta-acetyl-lup-20(29)-en-28-oic acid, 3-oxolup-20(29)-en-28-oic acid, and platanic acid.