Fermentation broths of Cryptosporiopsis sp. and Pezicula sp., endophytic fungi isolated from twigs of Pinus sylvestris and Fagus sylvatica respectively, yielded the same echinocandin compound L-671,329 as produced by Zalerion arboricola. Both fungi produced L-671,329 when grown in vitro. The compound was isolated and purified via chromatography, with a molecular weight of 1078 determined by mass spectral studies and a structure (assigned based on ¹H spectra in CD₃OD) consistent with data for L-671,329 published by Wichmann, Leisch & Schwartz (1989). Minimum inhibitory concentrations against Candida albicans and Saccharomyces cerevisiae were 0.015 and 2.0 μg ml⁻¹ respectively, while no activity was observed against Staphylococcus aureus, Escherichia coli, or Aspergillus spp. at 31 μg ml⁻¹. This study is the first to demonstrate that L-671,329 can be produced by Pezicula sp. and Cryptosporiopsis sp. in addition to Zalerion arboricola. It also highlights that some endophytic fungi (which cause symptomless infections in vascular plants) produce potent antifungal compounds that may confer a competitive advantage over other fungal colonizers, though their ability to produce echinocandins in vivo remains unproven.