Four novel oxygenated desmosterols, 24 zeta-hydroperoxy-6 beta-hydroxycholesta-4,25-dien-3-one (1), 25-hydroperoxy-6 beta-hydroxycholesta-4,23(E)-dien-3-one (2), 24 zeta-hydroperoxycholesta-4,25-diene-3,6-dione (3), and 25-hydroperoxycholesta-4,23(E)-diene-3,6-dione (4) were isolated from the marine red alga Galaxaura marginata. Steroids 1-4 and three synthetic oxygenated desmosterols 5-7 were shown to exhibit significant cytotoxicity against several cancer cell lines.