A new coumarin, isoarnottinin, and two new glucosides of isoarnottinin were isolated from the fruits of Ammi majus and characterized. Moreover, seven known coumarins, scopoletin, 8-(2''-acetoxy-3''-hydroxy-3''-methylbutoxy)psoralen, 8-hydroxy-5-methoxypsoralene, apiumetin, dihydroxanthyletin, lomatin and ammirol, as well as oleanolic acid and mannitol were found. Their structures were elucidated mainly on the basis of one- and two-dimensional NMR spectroscopy.