Micromonospora sp. CPCC 202787 is a strain isolated from soil, its EtOAc-soluble fraction of the extract from mycelia displayed significant inhibitory effect on HIV-1 replication in SupT1 Cells. That motivated us, and finally we obtained two new linear surfactins (1 and 2) and eight known cyclic surfactins (3 − 10) (Figure 1). Surfactin, a kind of peptide consisting of seven amino acids and one 3β-hydroxy fatty acids, is not only supposed to be the effective lipopeptide biosurfactant, but also to be bioactive constituent deserving investigation due to its multiple bioactivities such as antibacterial, antiviral, antitumor and hemolytic etc. 1 The linear surfactins have been reported for their surfactant property,2–4 whereas their NMR data and bioactivity apart from anti-TB5–7 are underreported.