The ester of N-benzoylphenylalanine and N-benzoylphenylalaninol, asperphenamate, was isolated from solid cultures of Penicillium brevicompactum. Isotope from L-[U-¹⁴C]phenylalanine was well incorporated into both benzoyl groups and into the phenylalanine and phenylalaninol moieties. Isotope from [U-¹⁴C]benzoic acid was also well incorporated into asperphenamate.