Alkaloids found in Picea breweriana include hygrine, hygroline, N-methylsedridine and N-methylpelletierine. This represents the first isolation of these alkaloids from the Pinaceae. Euphococcinine, an alkaloid previously found in Picea and Pinus species, is also present. The presence of alkaloids characteristic of two different biosynthetic pathways in P. breweriana highlights the apparent uniqueness of this species.