Nitraminoacetic acid, a substance with bacteriostatic activity, was isolated from the culture filtrate of Streptomyces noursei 8054-MC3. It showed a partial inhibition against some gram-negative bacteria, Escherichia coli, Xanthomonas oryzae and Pseudomonas tabaci, and exhibited inhibition against Mycobacterium phlei. It had been synthesized by Hantzsch and Metcalf1}, but there were no reports on its biological properties. Studies on the taxonomical properties of the producing organism, fermentation, isolation, physicochemical and biological properties and chemical synthesis of the active substance are described in this paper.