A mixture of acylglucosylsterols was isolated from the green fruits of Momordica charantia (balsam pear or bitter gourd) and the structure elucidated by high field ~HNMR, ]3CNMR, FTIR and mass spectrometry and chemical modification studies followed by spectral and chromatographic analysis The major acylglucosyl sterol was 3- O-[6'-O-palmitoyl-~-D-glucosyl]-stigmasta-5,25(27)-diene while the minor component was 3-O-[6'-O-stearyl-fl-D-glucosyl]-stigmasta-5,25(27)-diene. The isolation and structure elucidation of these acylglucosyl sterols are reported for the first time.