<jats:p>From the corms and seeds of Indian <jats:italic>Gloriosa superba</jats:italic> L., 19 tropolone and 3 non-tropolone alkaloids were isolated. Of these, cornigerine, (<jats:italic>S</jats:italic>)-(+)-floramultine (bechuanine), 1,12-dihydroxy-2,10,11-trimethoxyhomoaporphine, colchicamide, 2-demethylcolchifoline, 3-demethylcolchifoline, colchicoside, and isoperlolyrine are reported for the first time from this plant. The new alkaloid isoperlolyrine has been assigned the structure <jats:italic>III</jats:italic>. This is the first alkaloid of carboline type found in the subfamily <jats:italic>Wurmbaeoideae</jats:italic>.