The mother solution from the combined alkaloids of Peganum harmala (the plant was collected in May, 1973 in the building stage), after the isolation of the peganine and deoxypeganine [1, 2] was separated according to basicities on an apparatus for automatic liquid extraction [3] between buffer solutions of various pH values and chloroform. The chloroform mother solution (4 liters) contained 400 g of combined alkaloids. The solution of the combined alkaloids, and also the washing chloroform, was passed through the column of the apparatus at the rate of 1000-1200 ml/h, and then the buffer solutions were washed with pure chloroform. Each buffer solution was decanted off separately and made alkaline with concentrated ammonia to pH 8-9, and the alkaloids were extracted with chloroform. Then the chloroform was evaporated to dryness. The fractions of alkaloids obtained in this way were analyzed to TLC [nonfixed alumina; chloroform-methanol-benzene (5:1:4)], and the fractions with identical chromatograms were combined. The alkaloids were isolated according to their solubilities, by recrystallization, by the preparation of salts, by precipitation, etc. [2]. Thus, the total alkaloids of Peganum harmala contain strongly basic (pH of the extracting buffer 6.5-7.0), moderately basic (pH 3-6.5), and weakly basic (pH 2 and 10% H2SO4) alkaloids, additional amounts of which can be obtained by using polybuffer separation.