Amatoxins and phallotoxins are toxic cyclopeptides which can be found in Amanita phalloides. Most of the previous studies on the determination of these two families of toxins were carried out in pooled material by means of different analytical methods (1, 2, 4, 8, 13, 14). So far, only a few papers have been dedicated to the toxin content of single specimens (3, 9, 17). A detailed analysis has been described (9); using a combination of chromatographic separation with spectrophotometric determination, up to five amatoxins with six phallotoxins can be identified in a single A. phalloides mushroom. But this method is time-consuming and besides it seems to be unsuitable for application to large numbers of specimens. Recently, a high-performance liquid chromatographic assay (HPLC) was developed allowing, in a single specimen of mushroom, the assay of the main amatoxins, a-amanitin (a-Ama), β-amanitin (β-Ama) and γ-amanitin (γ-Ama), simultaneously with the main phallotoxins, phallisacin (PSC), phallacidin (PCD), phallisin (PHS), phalloidin (PHD) and phalloin (PHN) (7). The sensitivity, specificity and rapidity of this method prompted us to make use of it in a systematic search for amatoxins and phallotoxins among Amanita species. This study uses HPLC to detect amatoxins and phallotoxins in Amanita species of the subgenera Amanitopsis, Amanita, and Lepidella. It also reports variations in the amount and distribution of the toxins found in A. phalloides specimens. Finally, the identification and assay of amatoxins and phallotoxins in A. phalloides var. alba can be achieved.