A new 4-hydroxycoumarin glycoside (V), named 5-methyl-4-rutinosyloxycoumarin, C₂₂H₂₈O₁₂, mp 240—241 °C (dec.), [α]₂₂ - 79.4 ° (pyridine), was isolated from the underground parts of Gerbera jamesonii hybrida (Compositae), in addition to three cyanogenic glycosides (prunasin (I), amygdalin (II) and vicianin (III)) and a coumarin glycoside (4-β)-glucopyranosyloxy-5-methylcoumarin (IV)). The chemical structure of V was determined to be 5-methyl-4-[α-L-rhamnopyranopyranosyl-(1→6)-β-D-glucopyranosyloxyloumarin on the basis of chemical and spectral evidence.